Recombinant Mouse RANKL/TNFSF11 Protein (His Tag)

SKU: PKSM041165

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: RANKL;TNFSF11

Target Synonym: CD254;ODF;OPGL;Osteoclast differentiation factor;Osteoprotegerin ligand;RANKL;Receptor activator of nuclear factor kappa-B ligand;TNF-related activation-induced cytokine;TRANCE;Tnfsf11;Tumor necrosis factor ligand superfamily member 11

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: BLI

Fusion tag: N-His

UNIProt ID: O35235

Accession: BAA97257.1

Background: Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.

Concentration:

Activity: Loaded Recombinant Human OPG-Fc on Pro A Biosensor, can bind Mouse RANKL-His with an affinity constant of 1.02 pM as determined in BLI assay.

Sequence: Arg43-Asp287

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM HEPES-NaOH, 50mM NaCl, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 28.3 kDa

ObservedMW: 30-35 kDa