Recombinant Mouse NOV protein

SKU: CD00283

Size:5 ug

Background:NOV is a member of the CCN family of secreted cysteine rich regulatory proteins. The full length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm’s tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorgenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell surface integrins and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities. Recombinant Mouse NOV is a 36.4 kDa protein containing 333 amino acid residues. It is composed of four distinct structural domains (modules) - the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Nephroblastoma-overexpressed Gene Protein Homolog

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 1. μg/ml, corresponding to a specific activity of > 1 IU/mg.

Synonyms:Ccn3,C1388N23Rik, CCN,NOV, Mouse

Endotoxin:Less than 0.1 EU/μg of rMuNOV as determined by LAL method.

Storage:-20ºC

Sequence:QVSASLRCPS RCPPKCPSIS PTCAPGVRSV LDGCSCCPVC ARQRGESCSE MRPCDQSSGL YCDRSADPNN QTGICMVPEG DNCVFDGVIY RNGEKFEPNC QYFCTCRDGQ IGCLPRCQLD VLLPGPDCPA PRKVAVPGEC CEKWTCGSDE QGTQGTLGGL ALPAYRPEAT VGVEVSDSSI NCIEQTTEWS ACSKSCGMGV STRVTNRNRQ CEMVKQTRLC IVRPCEQEPE EV

uniprot accession:Q64299

Molecular weight:Approximately 36.4 kDa, a single non-glycosylated polypeptide chain containing 333 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM Tris-HCl, pH 8.6, 15 mM NaCl.