Recombinant Mouse MCP-5/CCL12 protein

SKU: CD00306

Size:5 ug

Background:Mouse CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with Mouse CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Monocyte Chemotactic Protein-5/CCL12

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1-5 ng/ml.

Synonyms:MCP-5/CCL12, MCP-, MCP-5, Scya, Scya12,Mouse

Endotoxin:Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.

Storage:-20ºC

Sequence:GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG

uniprot accession:Q6241

Molecular weight:Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.