Recombinant Mouse IL-36RA protein(His Tag)

SKU: PKSM041482

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-36RA

Target Synonym: EBI;Interleukin;Il1f;Il1h;Il1hy;EBI3;EBV-induced gene 3 protein;Epstein-Barr virus-in duced gene 3 protein;IL-27-A;IL-27B;IL-30;Interleukin-27 subunit alpha;Interleukin-27 subunit beta;Interleukin-30;p28;Fil1d;Il1f5;Il1h3;Il1hy1;IL36RN

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: Q9QYY1

Accession: Q9QYY1

Background: Human Interleukin-36 Receptor Antagonist (IL-36RN) is a secreted protein which belongs to the Interleukin 1 cytokine family (IL-1 family). IL-36RN is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RN is also detected in the spleen, brain leukocyte and macrophage cell types. Increased in lesional psoriasis skin. IL-36RN is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to Interleukin 1 family member 9 (IL1F9). Dysregulated expression of novel agonistic and antagonistic IL-1 family member ligands can promote cutaneous inflammation, revealing potential novel targets for the treatment of inflammatory skin disorders. Human and mouse IL-36RN share 90% sequence identity.

Concentration:

Activity: Measure by its ability to inhibit IL-36 gamma-induced IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <2 μg/mL.

Sequence: Met 2-Asp 156

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 17.8 kDa

ObservedMW: 17-25 kDa