Recombinant Mouse HB-EGF protein

SKU: CD00419

Size:10 ug

Background:Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. The protein is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Mouse HB-EGF shares about 81 % a.a. sequence identity with human HB-EGF.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Heparin-binding EGF-like Growth Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:HB-EGF,AW47313, Dt, Dtr, Dts, Hegf, Hegfl,HBEGF, Mouse

Endotoxin:Less than 1 EU/μg of rMuHB-EGF as determined by LAL method.

Storage:-20ºC

Sequence:DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL

uniprot accession:Q6186

Molecular weight:Approximately 9.8 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 1 mM PB, 5 mM NaCl, pH7.4.