Recombinant Human Wnt3a/Wnt-3a

SKU: PKSH033972

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: Wnt3a;Wnt-3a

Target Synonym: MGC119418;MGC119419;MGC119420;Wnt-3a;Wnt3a;member 3A;protein Wnt-3a;wingless-type MMTV integration site family

Research Areas:

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Cell culture

Fusion tag: None

UNIProt ID:

Accession:

Background: Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes.Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).

Concentration:

Activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is 71.45 ng/ml.

Sequence:

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.

Reconstitution: Not Applicable

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Calculated MW: 58.5 kDa

ObservedMW: 66-73 kDa