Recombinant Human UCHL1/PGP9.5 Protein (His Tag)

SKU: PKSH033175

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: UCHL1;PGP9.5

Target Synonym: HEL-117;NDGOA;Neuron Cytoplasmic Protein 9.5;PARK5;PGP 9.5;PGP9.5;UCH-L1;UCHL1;Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1;Ubiquitin Thioesterase L1;Uch-L1

Research Areas: Tags & Cell Markers;Cell biology;Neuroscience

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID: P09936

Accession: P09936

Background: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 (UCHL1) belongs to the Peptidase C12 family. UCHL1 is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ala223

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.9 kDa

ObservedMW: 26 kDa