Recombinant Human UBE2B protein (His Tag)

SKU: CD00730

Size:10 ug

Background:Ubiquitin-conjugating enzyme E2 B belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2B gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2B interacts with RAD18, UBR2 and WAC. Its protein sequence is 100 % identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Research_area:Others

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Ubiquitin Conjugating Enzyme E2 B, His

Biological activity:Data is not available.

Synonyms:UBE2B, E2-17kDa, HHR6B, HR6B, RAD6B, UBC2, Human

Endotoxin:Less than 1 EU/μg of rHuUBE2B, His as determined by LAL method.

Storage:-20ºC

Sequence:MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS

uniprot accession:P63146

Molecular weight:Approximately 19. kDa, a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a. vector sequence including 6 × His tag at N-terminus.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.