Recombinant Human TNF-α Protein(Gst Tag)

SKU: PDEH100596

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Cytokines

Abbreviation:TNF-α

Target Synonym:Tumor Necrosis Factor Ligand Superfamily Member;TNFSF;TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha;Cachectin;Tumor Necrosis Factor;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;APC1;APC1 protein;DIF、TNF-alpha、TNFA、TNFSF2;Differentiation inducing factor;Macrophage cytotoxic factor;macrophage derived;member 2;Membrane Form;monocyte derived;soluble form;sTNFA;TNF superfamily;Tumor necrosis factor alpha

Research Areas:Immunology;Signal Transduction;Cancer;Cardiovascular;Metabolism

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Gst

UNIProt ID:P01375

Accession:P01375

Background:Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1, CD120a, p55/60) and TNF-R2 (TNF receptor type 2, CD120b, p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Concentration:

Activity:Not validated for activity

Sequence:Val77-Leu233

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:43 kDa

ObservedMW:45 kDa