Recombinant Human TLK1/PKU-beta Protein (His & GST Tag)

SKU: PKSH030339

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: KIAA0137;PKU-beta

Research Areas: Epigenetics and Nuclear Signaling,Signal Transduction

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion tag: N-His-GST

UNIProt ID: Q9UKI8

Accession: Q9UKI8-1

Background: The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene.

Concentration:

Activity: The specific activity was determined to be 1. 6 nmol/min/mg using Histone H3 protein as substrate.

Sequence: Met 1-Tyr 766

Purity: > 88 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 114 kDa

ObservedMW: 125 kDa