Recombinant Human TFF1 protein

SKU: CD00834

Size:5 ug

Background:Trefoil factor 1 belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). TFF1 is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. It is studied because of commonly expressed in tumors as well.

Research_area: Signal Transduction

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Trefoil Factor 1

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 1 μg/ml, corresponding to a specific activity of ＞ 1 IU/mg.

Synonyms:TFF1,BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2, Human

Endotoxin:Less than 1 EU/μg of rHuTFF1 as determined by LAL method.

Storage:-20ºC

Sequence:EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF

uniprot accession:P4155

Molecular weight:Approximately 13.2 kDa, a homodimer consisting of two 6 amino acid polypeptide chains, which includes a 4-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, pH 7.4, 15 mM NaCl.