Recombinant Human SMAD3 Protein (His & Flag Tag)

SKU: PKSH032763

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: SMAD3

Target Synonym: JV15-2;MAD homolog 3;MADH3;Mad3;Mothers against DPP homolog 3;Mothers against decapentaplegic homolog 3;SMAD 3;SMAD family member 3;SMAD3;Smad3;hMAD-3;hSMAD3

Research Areas: Signal Transduction;Cancer;epigenetics and nuclear signal;metabolism;Stem cells

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His-Flag

UNIProt ID: P84022

Accession: P84022

Background: Mothers against decapentaplegic homolog 3(SMAD3) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD3 binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. It also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.

Concentration:

Activity: Not validated for activity

Sequence: Ser2-Ser425

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, 2mM EDTA, pH 8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 50.5 kDa

ObservedMW: 50-60 kDa