Recombinant Human Semaphorin 3A/SEMA3A Protein (His & Flag Tag)

SKU: PKSH033535

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Semaphorin 3A;SEMA3A

Target Synonym: Hsema-I;Hsema-III;SEMA1;SEMA3A;SEMAD;SemD;Sema III;Semad;Semaphorin III;coll-1

Research Areas: Cardiovascular;Neuroscience

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: N-His-Flag

UNIProt ID: Q14563

Accession: Q14563

Background: emaphorin-3A is a secreted protein which belongs to the semaphorin family. Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain.This protein can function as either a chemorepulsive agent; inhibiting axonal outgrowth; or as a chemoattractive agent; stimulating the growth of apical dendrites. In both cases; the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines.

Concentration:

Activity: Not validated for activity

Sequence: Lys26-Phe546

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 8.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 61.3 kDa

ObservedMW: 70-75 kDa