Recombinant Human RPE Protein (His Tag)

SKU: PKSH030953

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: HUSSY-17;RPE;RPE2-1;Ribulose-5-Phosphate-3-Epimerase;Ribulose-Phosphate 3-Epimerase

Research Areas: Signal Transduction;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: N-His

UNIProt ID: Q96AT9

Accession: NP_954699.1

Background: The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE); orotidine 5'-monophosphate decarboxylase (OMPDC); and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation; or origin; site; at which DNA synthesis begins. In enzymology; a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence; RPE has one substrate; L-ribulose 5-phosphate; and one product; L-xylulose 5-phosphate. RPE belongs to the family of isomerases; specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase; UlaE; and SgaU.

Concentration:

Activity: Not validated for activity

Sequence: Ala 2-Arg 228

Purity: > 94 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 27.0 kDa

ObservedMW: 30 kDa