Recombinant Human RANKL/TNFSF11 Protein (His Tag)

SKU: PKSH032986

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: RANKL;TNFSF11

Target Synonym: CD254;ODF;OPGL;OPTB2;Osteoclast differentiation factor;RANKL;Receptor activator of nuclear factor kappa-B ligand;TNFSF11;hRANKL2;sOdf;tumor necrosis factor ligand superfamily member 11

Research Areas: Cardiovascular;immunology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: O14788

Accession: O14788

Background: CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.

Concentration:

Activity: Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED₅₀ for this effect is <10 ng/mL.

Sequence: Glu 143-Asp317

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 20.7 kDa

ObservedMW: 17 kDa