Recombinant Human PSA/KLK3 Protein (His Tag)

SKU: PKSH031436

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: APS;Gamma-Seminoprotein;Hk3;KLK2A1;Kallikrein-3;P-30 Antigen;PSA;Prostate-Specific Antigen;Semenogelase;Seminin

Research Areas: Signal Transduction;Tags & Cell Markers;Neuroscience;Cancer;immunology;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Enzyme

Fusion tag: C-His

UNIProt ID: P07288

Accession: P07288

Background: KLK3(Kallikrein 3); also known as Prostate Specific Antigen (PSA); kallikrein-related peptidase 3; Gamma-seminoprotein; is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Concentration:

Activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA). The specific activity is > 100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Sequence: Met 1-Pro 261

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 28.3 kDa

ObservedMW: 35 kDa