Recombinant Human PF-4/CXCL4 protein

SKU: CD01134

Size:5 ug

Background:Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The gene for human CXCL4 is located on human chromosome 4. This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant human CXCL4 contains 70 amino acids binds with high affinity to heparin. Specifically, Human and mouse CXCL4 share about a 60 % identity.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Platelet Factor-4/CXCL4

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.-1 ng/ml.

Synonyms:PF-4/CXCL4, CXCL4, PF-4, SCYB4,Human

Endotoxin:Less than 1 EU/μg of rHuPF-4/CXCL4 as determined by LAL method.

Storage:-20ºC

Sequence:EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES

uniprot accession:P2776

Molecular weight:Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 7 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, 1.5 M NaCl, pH 7.4.