Recombinant Human PDE9A Protein (His & GST Tag)

SKU: PKSH031015

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: HSPDE9A2

Research Areas: Signal Transduction;

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application:

Fusion tag: N-His-GST

UNIProt ID: O76083

Accession: O76083-2

Background: High affinity cGMP-specific 3';5'-cyclic phosphodiesterase 9A; also known as PDE9A; is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis; brain; small intestine; skeletal muscle; heart; lung; thymus; spleen; placenta; kidney; liver; pancreas; ovary and prostate) except blood. Highest levels of PDE9A is in brain; heart; kidney; spleen; prostate and colon. IsoformPDE9A12is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain; with the highest expression observed in cerebellar Purkinje cells.PDE9A is the only cGMP-specific PDE with significant expression in the forebrain; and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP; which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ala 533

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 5mM GSH, pH 7.4, 10% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 89.5 kDa

ObservedMW: 75 kDa