Recombinant Human OPG protein

SKU: CD01180

Size:10 ug

Background:Osteoprotegerin (OPG), also named osteoclastogenesis inhibitory factor (OCIF), and tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a TNFRSF11B-encoded protein in humans. OPG is a 401 a.a. basic glycoprotein which comprises 7 structural domains. It is either a 60 kDa monomer or a 120 kDa dimer linked by disulfide bridges. OPG acts as a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL) and inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro and may also play a role in preventing arterial calcification. OPG has been applied to decrease bone resorption in women with postmenopausal osteoporosis and in patients with lytic bone metastases. Mature human OPG shares 86 %, 87 %, 92 %, 92 % and 88 % amino acid sequence identity with mouse, rat, equine, canine and bovine OPG, respectively.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Osteoprotegerin

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by neutralizing the stimulation of U937 cells is less than 1 ng/ml, corresponding to a specific activity of > 1. × 15 IU/mg in the presence of 1 ng/mL soluble rHuRANKL (sRANKL).

Synonyms:TNFRSF11B,OCIF, OPG, PDB5, TR1, Human

Endotoxin:Less than 1 EU/ug of rHuOPG as determined by LAL method.

Storage:-20ºC

Sequence:ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQK

uniprot accession:O3

Molecular weight:Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB,15 mM NaCl, pH 6..