Recombinant Human MTHFS Protein (His Tag)

SKU: PKSH032029

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: MTHFS

Target Synonym: 5,10-methenyl-tetrahydrofolate synthetase;5-formyltetrahydrofolate cyclo-ligase;MTHFS;Methenyl-THF synthetase

Research Areas: Signal Transduction;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID: P49914

Accession: P49914

Background: 5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ala203

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM Nacl, 1mM DTT, 50% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 24.3 kDa

ObservedMW: 28 kDa