Recombinant Human/Mouse/Rat BDNF Protein

SKU: PKSH033808

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: BDNF

Target Synonym: Abrineurin;BDNF;Brain-Derived Neurotrophic Factor

Research Areas: Cardiovascular;Neuroscience;metabolism

Conjugation:

Target Species: Human/Mouse/Rat

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P23560

Accession: P23560

Background: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF; NT-3 and NT-4; BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor; also known as p75). BDNF promotes the survival; growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.

Concentration:

Activity: Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED₅₀ for this effect is <2 ng/mL.

Sequence: His129-Arg247

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 14.5 kDa

ObservedMW: 14 kDa