Recombinant Human MMP8/CLG1 Protein (His Tag)

SKU: PKSH031731

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CLG1;MMP-8;MMP8;Matrix metalloproteinase-8;Neutrophil collagenase;PMNL collagenase;PMNL-CL

Research Areas: Signal Transduction;Cell biology;Cardiovascular;Cancer;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Enzyme

Fusion tag: C-His

UNIProt ID: P22894

Accession: NP_002415.1

Background: Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Neutrophil collagenase, also known as Matrix metalloproteinase-8, MMP-8, and CLG1, is a member of the peptidase M10A family. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 in the tumour may have a protective effect against lymph node metastasis. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.

Concentration:

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog#27076). The specific activity is > 250 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by APMA for an activated form)

Sequence: Met 1-Gly 467

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 10mM CaCl2, 150mM NaCl, pH 7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 52.6 kDa

ObservedMW: 65-75 kDa