Recombinant Human MIF Protein(Trx Tag)

SKU: PDEH100643

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:MIF

Target Synonym:MIF;GIF;GLIF;MMIF;Glycosylation inhibiting factor;Glycosylation-inhibiting factor;L-dopachrome isomerase;L-dopachrome tautomerase;Macrophage migration inhibitory factor;MIF protein;Phenylpyruvate tautomerase;GLIF;Glycosylation inhibiting factor;L-dopachrome isomerase;L-dopachrome tautomerase;Macrophage migration inhibitory factor;MIF;MIF protein;MMIF;Phenylpyruvate tautomerase;CLA;CTAGE;Macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Research Areas:Immunology

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:P14174

Accession:P14174

Background:Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is a ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence sμggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of the central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread, and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important role in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.

Concentration:

Activity:Not validated for activity

Sequence:1Met-115Ala

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:32.6 kDa

ObservedMW:32 kDa