Recombinant Human MFG-E8/lactadherin/MFGE8 Protein (His Tag)

SKU: PKSH031387

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: BA46;EDIL1;HMFG;HsT19888;MFG-E8;MFGM;OAcGD3S;SED1;SPAG10;hP47

Research Areas: Microbiology,Cardiovascular,Developmental Biology

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Cell culture

Fusion tag: C-His

UNIProt ID: Q08431

Accession: Q08431-1

Background: MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.

Concentration:

Activity: When 5 x 10⁴ cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg/mL, 100 μL/well), 45-85% cells will adhere after 1 hour at 37℃.

Sequence: Met 1-Cys 387

Purity: > 80 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS，0.2M Arg，0.01% Tween-20, pH7.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 42 kDa

ObservedMW: 45 kDa