Recombinant Human MECP2 Protein (His Tag)

SKU: PKSH032751

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: MECP2

Target Synonym: MECP2;MeCp-2 protein;Methyl-CpG-binding protein 2

Research Areas: Neuroscience;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P51608

Accession: P51608

Background: The MeCP2 helps regulate gene activity (expression) by modifying chromatin, the complex of DNA and protein that packages DNA into chromosomes. The MeCP2 protein is present in cells throughout the body, although it is particularly abundant in brain cells.In the brain, the MeCP2 protein likely plays a role in maintaining connections (synapses) between neurons, where cell-to-cell communication occurs. The alternative splicing of proteins is critical for normal communication between neurons and may also be necessary for the function of other types of brain cells.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Ser486

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 8% Sucrose, 50mM NaCl, 0.02% Tween 80, pH 6.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 53.5 kDa

ObservedMW: 90 kDa