Recombinant Human LRAP/ERAP2 Protein (His Tag)

SKU: PKSH031454

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: FLJ23633;FLJ23701;FLJ23807;L-RAP;LRAP;LRAP.ERAP2

Research Areas: Cell biology;immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Enzyme

Fusion tag: N-His

UNIProt ID: B2R769

Accession: NP_071745.1

Background: Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.

Concentration:

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is > 50 pmoles/min/μg.

Sequence: Ala 56-Thr 960

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycerol
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 106 kDa

ObservedMW: 115-125 kDa