Recombinant Human IL1F5/IL36RN Protein

SKU: PKSH031850

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL1F5;IL36RN

Target Synonym: FIL;UNQ;PRO;IL1F;IL1L;IL1HY;IL1RP;PSORS;IL36RN;FIL1;FIL1(DELTA);FIL1D;IL-36Ra;IL1F5;IL1HY1;IL1L1;IL1RP3;IL36RA;PSORP;PSORS14;UNQ1896;PRO4342;FIL1 Delta;IL-1 Delta;IL-1F5;IL-1HY1;IL-1L1;IL-1ra Homolog 1;IL-1-Related Protein 3;IL-1RP3;Interleukin-1 Delta;Interleukin-1 Family Member 5;Interleukin-1 HY1;Interleukin-1 Receptor Antagonist Homolog 1;Interleukin-1-Like Protein 1;Interleukin-36 Receptor Antagonist Protein

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: Q9UBH0

Accession: Q9UBH0

Background: Interleukin-1 family member 5 (IL-1F5); also known as interleukin 36 receptor antagonist (IL36RA); is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family; member 6 (IL1F6). IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A); beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1); which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor; single Ig IL-1R-related molecule (SIGIRR)/TIR8; as the effects were not observed in SIGIRR−/− mice. In contrast to its effects in brain tissue; IL-1F5 did not attenuate LPS-induced changes; or up-regulated IL-4 in macrophages or dendritic cells; suggesting that the effect is confined to the brain.

Concentration:

Activity: Measure by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells.The ED₅₀ for this effect is <2 ng/mL in the presence of 500 ng/mL of recombinant human IL-36 gamma.

Sequence: Met 1-Asp155

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 17.8 kDa

ObservedMW: 17 kDa