Recombinant Human IL-1A/IL-1α Protein

SKU: PKSH033458

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-1A;IL-1α

Target Synonym: IL1F;IL1A;IL-1A;IL1;IL1-ALPHA;IL1F1;BAF;FAF;Hematopoietin 1;Hematopoietin-1;IL 1 alpha;IL 1A;IL1 ALPHA;IL-1 alpha;IL1-A;Interleukin 1 alpha;Interleukin1 alpha;Interleukin-1 alpha;LAF;LEM;Preinterleukin 1 alpha;Pro interleukin 1 alpha;Pro-Interleukin-1-Alpha

Research Areas: immunology;metabolism

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P01583

Accession: P01583

Background: Interleukin-1 alpha (IL1α) is a cytokine member of the interleukin-1 family. IL-1 consists of two distinct forms: IL1α and IL1β that recognize the same cell surface receptors but are distinct proteins with approximately 25% amino acid sequence identity. IL1α is constitutively produced by epithelial cells and plays an essential role in maintenance of skin barrier function. Upon stimulation, a wide variety of cells including osteoblasts, monocytes, macrophages can be induced to express IL1α. IL1α possesses a wide range of metabolic, physiological, haematopoietic activities, and is critically involved in the regulation of the immune responses and inflammatory responses.

Concentration:

Activity: Measure by its ability to induce D10.G4.1 cells proliferation.The ED₅₀ for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 alpha is approximately >1x10⁸IU/ mg.

Sequence: Ser113-Ala271

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19 kDa

ObservedMW: 17 kDa