Recombinant Human IL-12 protein

SKU: CD01568

Size:2 ug

Background:Interleukin-12 (IL-12), also known as NKSF or CLMF, is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and Mouse IL-12 share 70 % and 60 % amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the Mouse system.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:CHO

Full product name:Recombinant Human Interleukin-12

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using PHA-activated human T lymphoblasts is less than .5 ng/ml, corresponding to a specific activity of > 2. × 17 IU/mg.

Synonyms:IL-12, CLMF, IL-12A, NFSK, NKSF1, P35,Human

Endotoxin:Less than 1 EU/μg of rHuIL-12 as determined by LAL method.

Storage:-20ºC

Sequence:p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS； p4 Subunit: IWELKKDVYV

uniprot accession:P2946

Molecular weight:Approximately 75 kDa, consisting of a 36 amino acid residue p4 subunit and a 197 amino acid residue p35 subunit.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.