Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

SKU: P04141

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: Immunology

Uniprot ID: P04141

Gene Names: CSF2

Alternative Name(s): Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor (CSF); Molgramostin; Sargramostim

Abbreviation: Recombinant Human CSF2 protein (Active)

Organism: Homo sapiens (Human)

Source: Mammalian cell

Expression Region: 18-144aa

Protein Length: Full Length of Mature Protein

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

MW: 16.7 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Less than 1.0 EU/μg as determined by LAL method.

Biological_Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 28.1-63.8 pg/mL.

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Reference: GM-CSF Primes Proinflammatory Monocyte Responses in Ankylosing Spondylitis. Shi H., Chen L., Ridley A., Zaarour N., Brough I., Caucci C., Smith J.E., Bowness P. Front Immunol 11: 1520-1520 (2020)

Function: