Recombinant Human FLT-3/FLK-2 Protein (Fc Tag)

SKU: PKSH033543

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: FLT-3;FLK-2

Target Synonym: CD135;FL Cytokine Receptor;FLK-2;FLK2;FLT-3;FLT3;Fetal Liver Kinase-2;Fms-Like Tyrosine Kinase 3;Receptor-Type Tyrosine-Protein Kinase FLT3;STK-1;STK1;Stem Cell Tyrosine Kinase 1

Research Areas: Signal Transduction;immunology;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-Fc

UNIProt ID: P36888

Accession: AAI26351.1

Background: The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2and Stk-1is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

Concentration:

Activity: Not validated for activity

Sequence: Asn27-Asn541

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 85.3 kDa

ObservedMW: 120 kDa