Recombinant Human FGF-21 protein

SKU: CD01804

Size:5 ug

Background:Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21 contains a 28 a.a. signal sequence and a 181 a.a. mature region but show limited binding to heparin. In addition, Mature human FGF-21 respectively shows 81 % a.a. identity to Mouse and rat FGF-21, and is known to be active on Mouse cells.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Fibroblast Growth Factor-21

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than .5 μg/ml, corresponding to a specific activity of > 2. × 13 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 1 μg/ml of heparin.

Synonyms:FGF-21,FGF21, Human

Endotoxin:Less than 1 EU/μg of rHuFGF-21 as determined by LAL method.

Storage:-20ºC

Sequence:HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

uniprot accession:Q9NSA1

Molecular weight:Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.