Recombinant Human FBPase 1/FBP1 Protein (Human Cells, His Tag)

SKU: PKSH033277

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: FBPase 1;FBP1

Target Synonym: 6-bisphosphatase 1;6-bisphosphate 1-phosphohydrolase 1;D-fructose-1;FBP;FBPase 1;Fructose-1

Research Areas: Signal Transduction;Cancer;epigenetics and nuclear signal;metabolism;

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P09467

Accession: P09467

Background: Fructose-1;6-bisphosphatase 1(FBP1) is a homotetramer protein and belongs to the FBPase class 1 family. It involves in carbohydrate biosynthesis; gluconeogenesis pathway. FBP1 is a gluconeogenesis regulatory protein which catalyzes the hydrolysis of fructose 1;6-bisphosphate to fructose 6-phosphate and inorganic phosphate. FBP1 deficiency is associated with hypoglycemia and metabolic acidosis. FBP1 regulates mouse endogenous glucose production. FBP1 coupled with phosphofructokinase (PFK) takes part in the metabolism of pancreatic islet cells.

Concentration:

Activity: Not validated for activity

Sequence: Ala2-Gln338

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 1mMEDTA, 10% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 37.8 kDa

ObservedMW: 35-38 kDa