Recombinant Human DFFA Protein (His Tag)

SKU: PKSH032359

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: DFFA

Target Synonym: DFF-45;DFF1;DFF45;DFFA;DNA Fragmentation Factor 45 kDa Subunit;DNA Fragmentation Factor Subunit Alpha;H13;ICAD;Inhibitor of CAD

Research Areas: Cell biology;Cancer;epigenetics and nuclear signal;metabolism

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: N-His

UNIProt ID: O00273

Accession: AAH07721.1

Background: DNA Fragmentation Factor Subunit Alpha (DFFA). DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Thr331

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 38.7 kDa

ObservedMW: 40-50 kDa