Recombinant Human CD226/DNAM-1 Protein (His Tag)

SKU: PKSH033728

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: CD226;DNAM-1

Target Synonym: CD226;CD226 antigen;DNAM-1;DNAM1;DNAX accessory molecule 1

Research Areas: immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: Q15762

Accession: Q15762

Background: Human DNAX accessory molecule 1 (DNAM-1/CD226) is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature human DNAM-1 contains an extracellular domain (ECD) with two Ig-like C2-set domains and a cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155 and CD112. Ligation of DNAM-1 promotes the activation of NK cells; CD8+ T cells; and mast cells; dendritic cell maturation; megakaryocyte and activated platelet adhesion to vascular endothelial cells; and monocyte extravasation; it inhibits the forrmation of osteoclasts. Plateletendothelium; interactions mediated by DNAM-1; enable the metastasis of tumor cells to the lung.

Concentration:

Activity: Not validated for activity

Sequence: Glu19-Asn247

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 26.8 kDa

ObservedMW: 43-55 kDa