Recombinant Human CAMK2A/CAMKA Protein (GST Tag)

SKU: PKSH030403

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: CAMKA

Research Areas: Signal Transduction;Cardiovascular;Neuroscience;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion tag: N-GST

UNIProt ID: A8K161

Accession: NP_741960.1

Background: Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase Ⅱ is thought to be an important mediator of learning and memory and is also necessary for Ca2+ homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus.

Concentration:

Activity: The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

Sequence: Met 1-His 478

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM Reduced Glutathione, pH 8.0

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 80.3 kDa

ObservedMW: 80 kDa