Recombinant Human Betacellulin/BTC Protein(Sumo Tag)

SKU: PDEH100599

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Betacellulin/BTC

Target Synonym:BTC

Research Areas:Signal Transduction

Conjugation:

Target Species:Human

Expression Host:E.coli

Application:

Fusion tag:N-Sumo

UNIProt ID:P35070

Accession:P35070

Background:Betacellulin(Betacellulin/BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. Betacellulin/BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices, 3 residues)36% beta sheet (5 strands, 18 residues). Betacellulin/BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Concentration:

Activity:Not validated for activity

Sequence:Asp32-Tyr111

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:21.7 kDa

ObservedMW:32 kDa