Recombinant Human APE1/APE Protein

SKU: PKSH032090

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: APE1;APE

Target Synonym: AP Endonuclease 1;APE;APE-1REF-1;APE1;APEN;APEX;APEX Nuclease;APEX1;APX;Apurinic-Apyrimidinic Endonuclease 1;DNA-(Apurinic or Apyrimidinic Site) Lyase;HAP1;REF1;Redox Factor-1

Research Areas: Cardiovascular;epigenetics and nuclear signal

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: P27695

Accession: AAH02338.1

Background: Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases; APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors; which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors; such as c-Jun; c-Fos; NF-kB; p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.

Concentration:

Activity: Not validated for activity

Sequence: Pro2-Leu318

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 35.6 kDa

ObservedMW: 40 kDa