Recombinant Human ANPEP/CD13 Protein (603 Met/Ile, His Tag)

SKU: PKSH031912

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: ANPEP/CD13

Target Synonym: LAP;ANPEP;APN;CD13;GP150;LAP1;P150;PEPN;Aminopeptidase N;AP-M;AP-N;CD13 antigen;CD13APN;PEPNhAPN; gp150;Alanyl (membrane) aminopeptidase;Alanyl aminopeptidase;Aminopeptidase M;AMPN;AP M;AP N;CD 13;hAPN;LAP 1;Microsomal aminopeptidase;Myeloid plasma membrane glycoprotein CD13

Research Areas: immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: Enzyme

Fusion tag: C-His

UNIProt ID: P15144

Accession: NP_001141.2

Background: Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

Concentration:

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is > 2, 500 pmoles/min/μg.

Sequence: Lys 69-Lys 967，603 Met/Ile

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.3
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 104.2 kDa

ObservedMW: 135-140 kDa