Recombinant Human ALPP Protein(His Tag)

SKU: PDMH100160

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:ALPP

Target Synonym:Placental alkaline phosphatase;ALPP;ALP;PALP;PLAP;PLAP-1;alkaline phosphatase;placental;ALPP and PLAP;placental type;Alkaline phosphatase Regan isozyme;Placental alkaline phosphatase 1;alkaline phosphatase;ALP;PALP;placental;PLAP;PLAP-1;Alkaline phosphatase placental;Alkaline phosphatase placental type;Alp1;FLJ61142;Germ-cell alkaline phosphatase;nagao isozyme;OTTHUMP00000164354;placental heat-stable alkaline phosphatase;PLAP1;PPB1

Research Areas:Cell Biology;Metabolism

Conjugation:

Target Species:Human

Expression Host:Mammalian

Application:

Fusion tag:C-His

UNIProt ID:P05187

Accession:P05187

Background:Most importantly, placental alkaline phosphatase (ALPP), an ectoenzyme that locates on cell surface with catalytic domains outside the plasma membrane and is overexpressed on many cancer cells, dephosphorylate the d-tyrosine phosphates on the surface of the magnetic nanoparticle and enable the magnetic nanoparticles to adhere selectively to the cancer cells, such as HeLa cells. Placental alkaline phosphatase (PLAP), encoded by the ALPP gene, is produced by the fetal side of the placenta.

Concentration:

Activity:Not validated for activity

Sequence:Ile23-Asp506

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method

Calculated MW:53.1 kDa

ObservedMW:60-70 kDa