Recombinant Human AKT1/PKB/PKBα Protein (His Tag)

SKU: PKSH031439

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA

Research Areas: Signal Transduction;Neuroscience;Cancer;epigenetics and nuclear signal;metabolism;

Conjugation:

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P31749

Accession: NP_001014431.1

Background: v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.

Concentration:

Activity: 1. No Kinase Activity 2. Measured by its ability to bind biotinylated human CD136 in a functional ELISA.

Sequence: Met 1-Ala 480

Purity: > 87 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, 3mM DTT, 0.5mM PMSF, 5% Glycerol, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 57.0 kDa

ObservedMW: 57 kDa