Recombinant HIV p24 protein (C-6His)

SKU: PKSV030277

Size: 1mg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation: p24

Target Synonym: Capsid protein p24;Human immunodeficiency virus 1;Human immunodeficiency virus type 1 p24

Research Areas:

Conjugation:

Target Species: HIV

Expression Host: E.coli

Application:

Fusion tag: C-His

UNIProt ID: P12493

Accession: P12493

Background: The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly,budding maturation and infection stages of the viral replication cycle. During viral assembly,the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Concentration:

Activity: Not validated for activity

Sequence: Met142-Gly355

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Trehalose, 5% Mannitol,0.01% Tween 80, 1mM EDTA, pH 9.0.

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 24.6 kDa

ObservedMW: 25 kDa