Recombinant Histone H3 (NT) Monoclonal Antibody

SKU: AN301549L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Histone H3

Target Synonym:Histone H;H3C;H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A;H3C1;Histone H3.1;Histone H3/a;Histone H3/b;H3FL;HIST1H3B;H3FC;HIST1H3C;H3FB;HIST1H3D;H3FD;HIST1H3E;H3FI;HIST1H3F;H3FH;HIST1H3G;H3FK;HIST1H3H;H3FF;HIST1H3I;H3FJ;HIST1H3J;H3/A;H3FA;H3FB;H3FC;H3FD;H3FF;H3FH;H3FI;H3FJ;H3FK;H3FL;Hist1h3a;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;Histone H3.1;Histone H3/a;Histone H3/b;H3 histone family;H31;H3a;histone 1;Histone cluster 1;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;member A

Research Areas:Epigenetics and Nuclear Signaling;Isotype;Loading Controls

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF;FCM;ChIP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A248

UNIProt ID:P68431

Accession:

Background:Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Among the histone members, histone H3 is the most extensively modified and its amino-terminal tail structure has been shown to be especially critical for eukaryotic cells.

Concentration:1 mg/mL

Immunogen:Synthetic peptide corresponding to the N-terminus of human histone H3 protein

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:100-1:500;IF 1:50-1:200;FCM 1:50-1:100;ChIP 6 μg/5×10⁶ cells

Calculated MW:15 kDa

ObservedMW:15 kDa