Recombinant Histone H2B (Ubiquityl Lys120) Monoclonal Antibody

SKU: AN302107L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Histone H2B

Target Synonym:HIST1H2BG;H2B.1A;H2B/a;H2BFA;H2BFL;HIST1H2BC;H2BFH;HIST1H2BE;H2BFG;HIST1H2BF;H2BFK;HIST1H2BI;H2BC4;H2BC6;H2BC7;H2BC8;H2BC10

Research Areas:Epigenetics and Nuclear Signaling

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A831

UNIProt ID:P62807

Accession:

Background:Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a “trans-histone” modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity.

Concentration:1 mg/mL

Immunogen:Ubiquitinated human histone H2B (Lys120) peptide

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000;IHC 1:200-1:1000

Calculated MW:23 kDa

ObservedMW:23 kDa