Recombinant Danio rerio Beta-actin/ACTB protein (His Tag)

SKU: PDEO100020

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Beta-actin;ACTB

Target Synonym:A26C1A;A26C1B;ACTB;ACTBB;Actin;Actin beta;Actin cytoplasmic 1;Actx;BRWS1;Beta cytoskeletal actin;Beta-actin;E430023M04Rik;MGC128179;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1;b actin;cytoplasmic 1

Research Areas:

Conjugation:

Target Species:Danio rerio

Expression Host:E.coli

Application:Immunogen

Fusion tag:N-His

UNIProt ID:Q7ZVF9

Accession:Q7ZVF9

Background:Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G-and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA

Concentration:

Activity:Not validated for activity

Sequence:Met1-Phe375

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:43.1 kDa

ObservedMW:42-48 kDa