Recombinant CD3E Monoclonal Antibody

SKU: AN301993L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:CD3E

Target Synonym:T3E;T-cell surface antigen T3/Leu-4 epsilon chain;T-cell surface glycoprotein CD3 epsilon chain;Cd3e;T3;CD3epsilon

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Mouse

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A713

UNIProt ID:P22646

Accession:

Background:When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab.

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:2000-1:5000

Calculated MW:21 kDa

ObservedMW:22 kDa