Recombinant CD3D & CD3E Monoclonal Antibody

SKU: AN300594P

Size::20μL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:CD3D & CD3E

Target Synonym:IMD;CD3E;IMD18;T3E;TCRE;CD3e molecule;CD3 epsilon;T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;T3;CD_antigen: CD3e;epsilon;

Research Areas:Immunology;Stem Cells

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human

Application:IHC-P;FCM

Isotype:IgG

Clonality:Monoclonal

Clone NO.:12B12

UNIProt ID:P07766&P04234

Accession:

Background:The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

Concentration:1 mg/mL

Immunogen:Recombinant Human CD3D & CD3E Heterodimer protein

Buffer:0.2 μm filtered solution in PBS

Purification method:Protein A

Dilution:IHC-P 1:100-1:500;FCM 1:25-1:100

Calculated MW:

ObservedMW: