Recombinant Carassiusauratus Leptin Protein (His Tag)

SKU: PKSQ050091

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: Leptin

Target Synonym: LEP;Leptin;OB;OBS;Obese Protein;Obesity Factor

Research Areas:

Conjugation:

Target Species: Carassius auratus

Expression Host: P.Pichia

Application:

Fusion tag: N-His

UNIProt ID: B8YI02

Accession: B8YI02

Background: Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish.

Concentration:

Activity: Not validated for activity

Sequence: Pro22-Cys171

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 8% Trehalose, 4% Mannitol, 0.02% Tween 80 (w/v), pH 5.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.3 kDa

ObservedMW: 17 kDa