Recombinant ATG4B Monoclonal Antibody

SKU: AN301739L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:ATG4B

Target Synonym:AUTL;KIAA;ATG4B;APG4B;AUTL1;cysteine protease ATG4B;KIAA0943;APG4B;AUTL1;cysteine protease ATG4B;APG4 autophagy 4 homolog B;ATG4 autophagy related 4 homolog B (S. cerevisiae);AUT like 1 cysteine endopeptidase;AUT-like 1 cysteine endopeptidase;Autophagin 1;Autophagin-1;Autophagy related 4B cysteine peptidase;Autophagy related cysteine endopeptidase 1;Autophagy related protein 4 homolog B;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;hAPG4B;MGC1353

Research Areas:Cancer;Cell Biology;Cardiovascular;Metabolism

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;FCM

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A447

UNIProt ID:Q9Y4P1

Accession:

Background:ATG4B is a member of the autophagin protein family. Its cysteine protease activity is required for the cytoplasm to vacuole transport (Cvt) and autophagy. ATG4B cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.

Concentration:1 mg/mL

Immunogen:Recombinant human ATG4B fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:200-1:1000;FCM 1:50-1:100

Calculated MW:44 kDa

ObservedMW:44 kDa