Recombinant Aquifex aeolicus DNA mismatch repair protein MutS (mutS), Biotinylated

SKU: O66652

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: O66652

Gene Names: mutS

Alternative Name(s):

Abbreviation: Recombinant Aquifex aeolicus mutS protein, Biotinylated

Organism: Aquifex aeolicus (strain VF5)

Source: E.coli

Expression Region: 1-859aa

Protein Length: Full Length

Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged

Target Protein Sequence: MEKSEKELTPMLSQYHYFKNQYPDCLLLFRLGDFYELFYEDAYIGSKELGLVLTSRPAGKGKERIPMCGVPYHSANSYIAKLVNKGYKVAICEQVEDPSKAKGIVKREVVRVITPGTFFERDTGGLASLYKKGNHYYVGYLNLAVGEFLGAKVKIEELLDLLSKLNIKEILVKKGEKLPEELEKVLKVYVSELEEEFFEEGSEEILKDFGVLSLQAFGFEEDTYSLPLGAVYKYAKTTQKGYTPLIPRPKPYRDEGFVRLDIKAIKGLEILESLEGRKDISLFKVIDRTLTGMGRRRLKFRLLSPFRSREKIERIQEGVQELKENREALLKIRQILEGMADLERLVSKISSNMATPRELVYLKNSLKKVEELRLLLLELKAPIFKEILQNFEDTKKIINDIEKTLVEDPPLHVKEGGLIREGVNAYLDELRFIRDNAETYLREYEKKLRQETGIQSLKIGYNKVMGYYIEVTKPNLKYVPSYFRRRQTLSNSERFTTEELQRLEEKILSAQTRINDLEYELYKELRERVVKELDKVGNNASAVAEVDFIQSLAQIAYEKDWAKPQIHEGYELIIEEGRHPVIEEFVENYVPNDTKLDRDSFIHVITGPNMAGKSSYIRQVGVLTLLSHIGSFIPARRAKIPVVDALFTRIGSGDVLALGVSTFMNEMLEVSNILNNATEKSLVILDEVGRGTSTYDGIAISKAIVKYISEKLKAKTLLATHFLEITELEGKIEGVKNYHMEVEKTPEGIRFLYILKEGKAEGSFGIEVAKLAGLPEEVVEEARKILRELEEKENKKEDIVPLLEETFKKSEEAQRLEEYEEIIKKIEEIDIGNTTPLQALLILAELKKKCSFSKKESGA

MW: 146.1 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

Reference: "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392: 353-358(1998)

Function: